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急慢性特发性血小板减少性紫癜患者外周血淋巴细胞亚群的表达

作者：张红刘庆华田芳

来源：《中国实用医药》2014年第27期

【摘要】目的检测急慢性特发性血小板减少性紫癜（ITP）患者外周血淋巴细胞亚群，

并探讨其在发病机制中的作用。方法选择74例ITP患者及30例健康查体人员的外周血，采用流式细胞术检测淋巴细胞亚群（CD3+， CD3+CD4+， CD3+CD8+， CD4+/CD8+，

CD19+）。结果① ITP患者淋巴细胞亚群CD3+CD4+为（34.15±8.55）%，正常对照组为（36.74±3.65）%， t=2.166， P=0.033；ITP患者CD4+/CD8+为（1.24±0.44）%，正常对照组为（1.48±0.25）%， t=3.582， P=0.001；ITP患者CD3+CD8+为（30.63±7.72）%， CD19+为（18.23±7.67）%，均显著高于正常对照组（P=0.000）。②急性ITP患者外周血CD19+细胞比例为（22.6±7.25）%，慢性ITP患者为（14.14±5.57）%，两者差异有统计学意义

（t=5.677， P=0.000）；而慢性ITP患者的CD3+细胞的比例为（76.02±11.00）%，急性ITP

患者为（66.82±10.95）%， t=3.583， P=0.001。结论 ITP的发生不仅存在B淋巴细胞异常，

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也存在T淋巴细胞的异常。急性ITP患者以体液免疫功能亢进为主，慢性患者则表现为细胞

免疫功能亢进为主。

【关键词】特发性血小板减少性紫癜；淋巴细胞亚群；细胞免疫；体液免疫

【Abstract】 Objective To detect the expression of peripheral blood lymphocyte subpopulation in patients with acute and chronic idiopathic thrombocytopenic purpura （ITP）， and to investigate its influence on pathogenesis. Methods The expression of lymphocyte subpopulation （CD3+，

CD3+CD4+， CD3+CD8+， CD4+/CD8+， CD19+） in the peripheral blood was analyzed using flow cytometry in 74 patients with ITP and 30 healthy people. Results ① CD3+CD4+ of lymphocyte subpopulation in patients with ITP was （34.15±8.55）%， while that of the control group was （36.74±3.65）% （t=2.166， P=0.033）. CD4+/CD8+ of lymphocyte subpopulation in patients with ITP was （1.24±0.44）%， and that of the control group was （1.48±0.25）% （t=3.582，

P=0.001）. CD3+CD8+ and CD19+ of lymphocyte subpopulation in patients with ITP were

（30.63±7.72）% and （18.23±7.67） %， and they were all obviously higher than the control group （P=0.000）.② The proportion of CD19+ B cells in peripheral Blood of acute ITP patients was （22.6±7.25）%， and that was （14.14±5.57%） for chronic ITP patients. The difference had statistical significance （t=5.677， P=0.000）. The proportion of CD3+ T cells in chronic ITP patients was （76.02±11.00）%， while that in acute ITP patients was （66.82±10.95）%

（t=3.583， P=0.001）. Conclusion ITP patients are disorder with B and T lymphocyte. Acute patients always accompanied with hyperfunctioning of humoral immune， while the chronic ones were with hyperfunctioning of cellular immune.